Optimization of the URA-blaster disruption system in Candida guilliermondii: efficient gene targeting using the URA3
Emilien Foureau1, Vincent Courdavault, Andrew J Simkin
1EA2106, Biomolécules et Biotechnologies Végétales, UFR Pharmacie, Université François Rabelais de Tours, France.
Journal of Microbiological Methods
|August 14, 2012
Abstract:
We established a simple transformation system for C. guilliermondii by developing both an ura3 ATCC 6260-derived recipient strain as well as an URA3 blaster cassette. We demonstrated that this strategy allows efficient multiple gene disruption by homologous recombination with a convenient gene targeting frequency.
