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Related Experiment Video

Updated: May 19, 2026

AQRNA-seq for Quantifying Small RNAs

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing.

Markus Hafner¹, Neil Renwick, Thalia A Farazi

¹Laboratory of RNA Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 186, NY 10065, USA.

Methods (San Diego, Calif.)

|August 14, 2012

Summary

This summary is machine-generated.

Researchers developed a protocol for barcoded small RNA cDNA library preparation for Illumina sequencing. This method enables efficient profiling of microRNAs (miRNAs) and other small RNAs in large sample sets.

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Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

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Last Updated: May 19, 2026

AQRNA-seq for Quantifying Small RNAs

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

Area of Science:

Molecular Biology
Genomics
Post-transcriptional Regulation

Background:

Small regulatory RNAs, including microRNAs (miRNAs) and piwi-interacting RNAs (piRNAs), are crucial in post-transcriptional gene regulation.
Accurate identification and quantification of small RNAs are essential for understanding their roles in development and disease.
Characterizing small RNAs across diverse biological conditions requires robust and scalable methodologies.

Purpose of the Study:

To present a standardized protocol for generating barcoded small RNA cDNA libraries.
To enable high-throughput sequencing and profiling of small RNAs using Illumina HiSeq technology.
To facilitate comprehensive analysis of small RNA populations in various biological contexts.

Main Methods:

Development of a step-by-step protocol for small RNA cDNA library construction.
Incorporation of unique barcodes for multiplexing samples.
Adaptation of the protocol for compatibility with Illumina HiSeq sequencing platforms.

Main Results:

Successful generation of barcoded small RNA cDNA libraries.
Demonstration of the protocol's utility for profiling miRNAs and other small RNAs.
Facilitation of parallel processing of numerous samples for small RNA analysis.

Conclusions:

The presented protocol provides a reliable method for small RNA profiling.
This approach supports large-scale studies of small RNA expression in diverse biological samples.
The methodology aids in advancing the characterization of post-transcriptional gene regulation.