Pichia surface display: a tool for screening single domain antibodies

Area of Science:

• Biotechnology
• Molecular Biology
• Immunology

Background:

• Yeast surface display is a powerful technique for antibody discovery and engineering.
• Pichia pastoris is a widely used yeast for recombinant protein production.
• Single domain antibodies offer advantages in size and stability compared to traditional antibodies.

Purpose of the Study:

• To describe protocols for a novel yeast surface display system.
• To enable efficient isolation and engineering of single domain antibodies.
• To utilize Pichia pastoris for antibody display and selection.

Main Methods:

• Development of a yeast surface display system in Pichia pastoris.
• Fusion of single domain antibody libraries to Saccharomyces cerevisiae alpha-agglutinin gene (SAG1).
• Expression of antibody fragments on the P. pastoris cell surface.
• Ligand-based labeling for quantitative analysis and isolation.

Main Results:

• Successful establishment of a Pichia pastoris-based yeast surface display system.
• Demonstration of efficient display of single domain antibodies.
• Facilitation of rapid and quantitative isolation of antibodies with specific binding properties.

Conclusions:

• The Pichia pastoris yeast surface display system is an effective tool for antibody discovery.
• This platform allows for the isolation and engineering of single domain antibodies.
• The described protocols provide a robust method for selecting antibodies with desired characteristics.