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Related Concept Videos

Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Blood Studies for Cardiovascular System III: Serum Lipid Profile01:25

Blood Studies for Cardiovascular System III: Serum Lipid Profile

Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
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Related Experiment Video

Updated: May 19, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

High Density Lipoprotein and it's Dysfunction.

Esin Eren1, Necat Yilmaz, Ozgur Aydin

  • 1Antalya Public Health Center of Ministry of Health, Antalya, Turkey.

The Open Biochemistry Journal
|August 14, 2012
PubMed
Summary

High-density lipoprotein cholesterol (HDL-C) levels don't reflect HDL functionality. Enhancing HDL function, not just quantity, may be key for preventing cardiovascular disease (CVD).

Keywords:
HDLHDL dysfunctionHDL functionalityHDL proteinsHDL subtypes.High density lipoprotein

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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
06:47

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

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Last Updated: May 19, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
06:47

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Area of Science:

  • Biochemistry
  • Cardiovascular Medicine
  • Clinical Chemistry

Background:

  • Traditional lipid management focuses on lowering LDL-C and increasing HDL-C to prevent atherosclerosis and CVD.
  • However, HDL-C levels alone do not accurately predict HDL particle functionality or composition.
  • A significant portion of cardiovascular deaths remain unpreventable with current LDL-C lowering strategies, highlighting the need for alternative targets.

Purpose of the Study:

  • To review current knowledge on assays measuring HDL functionality.
  • To explore the relationship between HDL functions and cardiovascular disease (CVD).
  • To highlight HDL functionality as a potential therapeutic target for CVD prevention.

Main Methods:

  • Review of current scientific literature on HDL functionality assays.
  • Analysis of studies correlating HDL functions (e.g., cholesterol efflux, antioxidant, anti-inflammatory) with CVD risk.
  • Discussion of the role of clinical chemists in developing and validating new biomarkers.

Main Results:

  • HDL-C levels are insufficient predictors of HDL's role in preventing atherosclerosis.
  • HDL's key functions include promoting cholesterol efflux for reverse cholesterol transport (RCT).
  • Enhanced antioxidant and anti-inflammatory activities of HDL are linked to CVD protection.

Conclusions:

  • Focus is shifting from HDL quantity to HDL quality and functionality for CVD prevention.
  • Strategies to enhance HDL functionality offer a promising therapeutic avenue.
  • Development of validated HDL function assays is crucial for clinical application and public health.