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Related Concept Videos

Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Production of Formed Elements01:34

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

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Updated: May 19, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

Hematopoietic Progenitor Cell Apheresis processing.

Eleanor S Hamilton1, Edmund K Waller

  • 1Cellular Therapies Laboratory, Emory University Hospital, Atlanta, GA, USA. Ellie.hamilton@emoryhealthcare.org

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2012
PubMed
Summary
This summary is machine-generated.

Apheresis effectively collects hematopoietic stem and progenitor cells for cancer treatment. Freezing and thawing these cells maintains viability, enabling autologous transplants after intensive chemotherapy.

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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

Area of Science:

  • Hematology
  • Transplant Medicine
  • Cellular Therapy

Background:

  • Apheresis yields mononuclear cells enriched in hematopoietic stem and progenitor cells (HSPCs).
  • Mobilization of HSPCs is achieved through myeloid growth factors, CXCR4 antagonists, or chemotherapy recovery.
  • Autologous transplantation uses cryopreserved HSPC grafts following myeloablative therapy for cancer treatment.

Purpose of the Study:

  • To describe the process and factors influencing hematopoietic stem and progenitor cell collection via apheresis.
  • To highlight the significance of cryopreservation for autologous stem cell transplantation.

Main Methods:

  • Apheresis procedures are performed on patients mobilized with specific agents or during chemotherapy recovery.
  • Collection duration is determined by apheresis efficiency, target cell numbers (CD34+ cells), and patient weight.
  • Hematopoietic progenitor cell (HPC) grafts are cryopreserved to maintain cell viability.

Main Results:

  • Apheresis, under specific mobilization conditions, yields products highly enriched for HSPCs.
  • The efficiency of apheresis and patient-specific factors influence collection duration.
  • Cryopreservation successfully retains the viability of HSPCs in collected grafts.

Conclusions:

  • Apheresis is a key method for collecting HSPCs for autologous transplantation.
  • Cryopreservation technology is crucial for the routine use of autologous HPC grafts in cancer therapy.
  • This approach supports myeloablative chemotherapy and radiation regimens with stem cell rescue.