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Spectral encoding of spatial frequency approach for characterization of nanoscale structures

Sergey A Alexandrov1, Shikhar Uttam, Rajan K Bista

  • 1Biomedical Optical Imaging Laboratory (BOIL), Department of Medicine and Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15232, USA.

Applied Physics Letters
|August 16, 2012
PubMed

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Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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