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Related Experiment Video

Updated: May 19, 2026

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Published on: September 5, 2015

Measuring intracellular ion concentrations with multi-barrelled microelectrodes.

Anthony J Miller¹, Susan Smith

¹Department of Disease and Stress Biology, John Innes Centre, Norwich, UK. tony.miller@jic.ac.uk

Methods in Molecular Biology (Clifton, N.J.)

|August 17, 2012

Summary

Ion-selective microelectrodes are crucial for measuring intracellular ion concentrations and identifying cellular compartments. Using a membrane matrix is essential for plant cell measurements due to turgor pressure.

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Area of Science:

Electrochemistry
Plant Physiology
Cell Biology

Background:

Ion-selective microelectrodes are vital tools for quantifying intracellular ion concentrations.
Multi-barrelled electrodes allow for the differentiation of cellular compartments, such as distinguishing cytoplasm from vacuole using pH-selective electrodes.

Purpose of the Study:

To detail the composition and application of ion-selective microelectrodes.
To highlight the importance of membrane matrix inclusion for accurate measurements in plant cells.

Main Methods:

Microelectrodes are filled with sensor cocktails comprising ion-selective molecules, membrane solvents, additives, and a membrane matrix.
The use of a high molecular weight poly(vinyl chloride) or other polymers like nitrocellulose as a membrane matrix is discussed.

Main Results:

Sensor cocktails can be purchased pre-made or mixed individually for cost-effectiveness.
A membrane matrix is crucial for maintaining electrode sensitivity in plant cells by counteracting turgor pressure, which can otherwise displace liquid membranes.

Conclusions:

Ion-selective microelectrodes, particularly with a membrane matrix, provide reliable methods for intracellular ion analysis in various cell types, including plants.
DIY sensor cocktail preparation offers a cheaper alternative to commercial products, provided a suitable matrix is incorporated for plant applications.