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Updated: May 19, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single-Cell Sampling and Analysis (SiCSA).

Wieland Fricke¹

¹School of Biology and Environmental Science, University College Dublin, Dublin, Ireland. Wieland02fricke@yahoo.co.uk

Methods in Molecular Biology (Clifton, N.J.)

|August 17, 2012

Summary

This summary is machine-generated.

Single-cell sampling and analysis reveals solute concentrations within plant cells and tissues. This technique is crucial for understanding plant stress responses, especially salinity, by detailing ion distribution and its impact on survival.

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Area of Science:

Plant Physiology
Analytical Chemistry
Cell Biology

Background:

Understanding solute distribution at the cellular level is vital for plant stress research.
Salinity stress significantly impacts plant physiology, with ion distribution playing a critical role in survival.

Purpose of the Study:

To detail selected Single-Cell Sampling and Analysis (SiCSA) methods.
To discuss the advantages and potential limitations of these SiCSA techniques.

Main Methods:

Pressure-driven extraction of cell contents (cell sap sampling).
Analysis of extracted cell sap using picolitre osmometry for osmolality.
Energy-dispersive X-ray analysis for elemental concentrations (Na, K, P, S, Cl, Ca).
Microfluorometry for quantifying compounds like nitrate and total amino acids.

Main Results:

SiCSA methods enable precise measurement of solute concentrations in individual plant cells and tissues.
The described methods allow for detailed investigation of ion and metabolite distribution under stress conditions.

Conclusions:

SiCSA provides valuable insights into cell- and tissue-specific responses to environmental stresses like salinity.
These analytical methods are essential tools for advancing our understanding of plant stress physiology.