Recent advances in oligonucleotide synthesis and their applications
B Vaijayanthi1, P Kumar, P K Ghosh
1Institute of Genomics and Integrative Biology, Delhi University Campus, Delhi 110 007, India.
Indian Journal of Biochemistry & Biophysics
|August 21, 2012
Summary
Advancements in synthetic oligonucleotide chemistry enable efficient, cost-effective nucleic acid synthesis. Oligonucleotide conjugates and microarray technology expand applications in genomics and diagnostics.
Area of Science:
- Nucleic Acids Chemistry
- Genomics
- Medicinal Chemistry
Background:
- Chemical synthesis of nucleic acids has significantly advanced since the isolation of nucleic acids and human genome mapping.
- Key developments include high-throughput synthesizers, improved reagents, polymer supports, and protecting groups for nucleic bases.
Purpose of the Study:
- To review major developments in synthetic oligonucleotide chemistry.
- To highlight applications of oligonucleotide conjugates and microarray technology.
Main Methods:
- Review of advancements in oligonucleotide synthesis technologies.
- Discussion of oligonucleotide conjugation strategies.
- Overview of microarray technology applications.
Main Results:
- Significant improvements in efficiency, cost-effectiveness, and speed of oligonucleotide assembly.
- Identification of new applications through oligonucleotide conjugates with biological and abiological ligands.
- Demonstration of microarray technology's utility in genetic mutation screening and diagnostics.
Conclusions:
- Synthetic oligonucleotide chemistry has been revolutionized by technological advancements.
- Oligonucleotide conjugates and microarray technology offer diverse and expanding applications in various scientific fields.
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