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An improved method for quantitative determination of urinary porphyrins by use of second-derivative spectroscopy
A W van de Giessen1, E M van Wijk
1Clinical Chemistry Department, Military Hospital Dr. A. Mathijsen, Utrecht, The Netherlands.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|September 1, 1990
Abstract:
An improved assay for quantification of urinary porphyrins by use of second-derivative spectroscopy is described. A new method for calculation of the porphyrin concentration is developed and the whole procedure is computerized. Acidified urine samples can be assayed within a few minutes by using this method. Precision and recoveries for both uro- and coproporphyrin are good. The method is presented as a very fast and accurate assay for the screening and quantification of urinary porphyrins.