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Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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Reprogramming cell fates: insights from combinatorial approaches.

Carlos-Filipe Pereira1, Ihor R Lemischka, Kateri Moore

  • 1Department of Developmental and Regenerative Biology, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA. filipe.pereira@mssm.edu

Annals of the New York Academy of Sciences
|August 21, 2012
PubMed
Summary
This summary is machine-generated.

Transcription factors (TFs) can reprogram cells to new identities. Determining the minimal TF core network could enable direct cell conversion, advancing regenerative medicine and developmental biology.

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Area of Science:

  • Cellular reprogramming and developmental biology.
  • Epigenetics and gene regulation.
  • Regenerative medicine and cell therapy.

Background:

  • Epigenetic reprogramming is key to cell fate determination.
  • Methods include nuclear transfer, cell fusion, and transcription factor (TF) expression.
  • Combinatorial TF overexpression defines minimal networks for specific cell fates.

Purpose of the Study:

  • To review TFs used for reprogramming diverse cell identities.
  • To explore the concept of a common blueprint for reprogramming factors.
  • To discuss the potential of TF-mediated cell conversion for regenerative therapies and understanding development.

Main Methods:

  • Review of existing literature on transcription factor-mediated reprogramming.
  • Analysis of TF combinations used to induce pluripotency and differentiated cell types.
  • Conceptual framework for identifying minimal TF core networks.

Main Results:

  • TF-mediated reprogramming has successfully induced pluripotency and various differentiated cell types.
  • Reprogramming has been achieved across distant cell types, including those from different germ layers.
  • The study highlights the potential for direct cell-type conversion if the correct TF core is identified.

Conclusions:

  • TF-mediated cell conversion offers a direct route to generating specific cell types.
  • Identifying a common blueprint for reprogramming factors could revolutionize cell therapy.
  • This approach will significantly impact the understanding of biological development, particularly for somatic stem cells.