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Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Methods of Nuclear Reprogramming01:24

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Related Experiment Video

Updated: May 1, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening.

Abigail R Altman1, Diogo Pértiga-Cabral2, Carlos-Filipe Pereira3

  • 1Molecular Medicine and Gene Therapy, Science for Life Laboratory, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden.

STAR Protocols
|April 29, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a new protocol for screening transcription factor (TF) combinations to understand how they work together. This method helps map the networks that control cell identity for therapeutic cell generation.

Keywords:
BioinformaticsFlow CytometryImmunologyRNAseqSequencingSingle CellStem Cells

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Area of Science:

  • Cell biology
  • Molecular biology
  • Immunology

Background:

  • Direct reprogramming is a promising method for generating therapeutic cells.
  • Understanding transcription factor (TF) cooperativity is crucial for advancing reprogramming technologies.
  • Current knowledge of TF networks driving cell identity is incomplete.

Purpose of the Study:

  • To present a protocol for combinatorial TF screening.
  • To resolve the reprogramming factor networks that dictate cell identity.
  • To enable the generation of distinct immune cell types from human fibroblasts.

Main Methods:

  • Arrayed lentiviral production and transduction of human fibroblasts.
  • Combinatorial screening of transcription factors (TFs).
  • Cell purification, library preparation, sequencing, and data analysis for TF dynamics.

Main Results:

  • Detailed protocol for TF screening and network resolution.
  • Demonstration of reprogramming human fibroblasts into specific immune cells.
  • Identification of TF combinations and their dynamics in cell fate determination.

Conclusions:

  • The developed protocol facilitates the systematic investigation of TF cooperativity.
  • This approach can accelerate the discovery of novel reprogramming strategies.
  • Advances understanding of cell identity control for therapeutic applications.