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Hadas Ben-Gera1, Naomi Ori

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Summary
This summary is machine-generated.

Tomato leaf complexity is regulated by auxin signaling and the LANCEOLATE (LA) gene. Auxin and ENTIRE (E) influence leaflet initiation, but have minor effects on indeterminate leaf growth.

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Area of Science:

  • Plant Developmental Biology
  • Molecular Genetics
  • Plant Physiology

Background:

  • Leaf complexity in plants depends on the marginal-blastozone (MB) tissue.
  • In tomato (Solanum lycopersicum), the TCP transcription factor LANCEOLATE (LA) restricts MB activity, influencing leaf shape.
  • Auxin signaling, marked by DR5::VENUS, is crucial for leaflet initiation, and ENTIRE (E), an auxin signal inhibitor, affects leaf morphology.

Purpose of the Study:

  • To investigate the interplay between auxin, ENTIRE (E), and LANCEOLATE (LA) in tomato leaf development.
  • To understand how these factors regulate leaflet initiation and indeterminate growth at the leaf margin.

Main Methods:

  • Analysis of tomato mutants including La-2 (dominant LA mutant), pFIL>>miR319 (overexpressing miR319), and e (entire mutant).
  • Utilized DR5::VENUS auxin response sensor to visualize auxin signaling.
  • Examined double mutants (e La-2) and analyzed leaf phenotypes and leaflet initiation.

Main Results:

  • In La-2 mutants, auxin signaling is weak and diffused, indicating auxin acts within the LA-controlled MB activity context.
  • e La-2 double mutants exhibit an enhanced simple leaf phenotype.
  • pFIL>>miR319 leaves show reduced leaflet initiation but continuous marginal growth, suggesting E and auxin primarily affect initiation, not indeterminate growth.

Conclusions:

  • Auxin and ENTIRE (E) modulate leaflet initiation within the framework of extended marginal-blastozone (MB) activity.
  • The influence of E and auxin on the extent of indeterminate leaf growth is limited.
  • LA plays a key role in restricting MB activity and thus controlling leaf complexity.