CLASS-II KNOX genes coordinate spatial and temporal ripening in tomato.
Alexandra Keren-Keiserman1, Amit Shtern1, Matan Levy2
1Institute of Plant Sciences, ARO, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel.
Plant Physiology
|June 15, 2022
Summary
CLASS-II KNOX genes coordinate fruit maturation in dry and fleshy fruits. In tomato, these genes control spatial ripening patterns by differentially regulating the RIPENING INHIBITOR (RIN) gene.
Area of Science:
- Plant Molecular Biology
- Fruit Development and Ripening
Background:
- Fruits mature via senescence (dry) or ripening (fleshy), with partially shared molecular networks.
- CLASS-II KNOX genes regulate dry fruit senescence, but their role in fleshy fruit development is unknown.
Purpose of the Study:
- Investigate the function of tomato CLASS-II KNOX (TKN-II) genes in fleshy fruit ripening.
- Determine if TKN-II genes contribute to conserved fruit maturation pathways.
Main Methods:
- Utilized knockout alleles of individual TKN-II genes in tomato (Solanum lycopersicum).
- Created an artificial microRNA line (35S:amiR-TKN-II) to simultaneously target all TKN-II genes.
- Analyzed fruit size, ripening phenotypes, and expression of the ripening marker gene RIPENING INHIBITOR (RIN).
Main Results:
- TKN-II gene disruption resulted in smaller tomato fruits.
- Simultaneous TKN-II gene knockdown led to precocious ripening in the locular domain but stalled pericarp ripening.
- TKN-II genes appear to regulate RIN activity spatially, inhibiting it in the locular domain and promoting it in the pericarp.
Conclusions:
- CLASS-II KNOX genes play a redundant role in coordinating maturation across dry and fleshy fruits.
- In tomato, TKN-II genes control spatial patterns of fruit ripening through differential regulation of RIN activity.
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