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Related Experiment Video

Updated: May 19, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

Eclipta prostrata L. phytochemicals: isolation, structure elucidation, and their antitumor activity.

Qi-Mei Liu1, Hai-Yan Zhao, Xian-Ke Zhong

  • 1College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
|August 21, 2012
PubMed
Summary

Eclipta prostrata extracts show significant antitumor potential. A 30% ethanol fraction and eclalbasaponin I notably inhibited hepatoma cell proliferation, outperforming the positive control.

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Last Updated: May 19, 2026

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09:33

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Published on: March 20, 2018

Area of Science:

  • Phytochemistry
  • Pharmacology
  • Natural Products

Background:

  • Eclipta prostrata (Asteraceae) is traditionally used in China for medicinal purposes.
  • Research focuses on identifying bioactive compounds from E. prostrata for therapeutic applications.

Purpose of the Study:

  • To isolate and purify phytochemical constituents from E. prostrata.
  • To evaluate the in vitro antitumor activity of extracts and isolated compounds against hepatoma cells.

Main Methods:

  • Phytochemical isolation using gradient solvent fractionation, macroporous resin, silica gel, Sephadex LH-20, ODS columns, and TLC.
  • Structure identification of compounds via MS, (1)H, and (13)C NMR spectroscopy.
  • In vitro antitumor activity assay using hepatoma cell line smmc-7721.

Main Results:

  • Four compounds were isolated: wedelolactone, eclalbasaponin I, luteolin, and luteolin-7-O-glucoside.
  • The 30% ethanol fraction exhibited potent dose-dependent inhibition of hepatoma cell proliferation (IC50 = 74.24 μg/ml).
  • Eclalbasaponin I also showed significant inhibition (IC50 = 111.17 μg/ml), both exceeding the positive control 5-fluorouracil (IC50 = 195.31 μg/ml).

Conclusions:

  • Eclipta prostrata extracts, particularly the 30% ethanol fraction and eclalbasaponin I, possess significant antitumor activity.
  • These findings suggest E. prostrata is a promising source for developing novel natural anti-tumor agents.