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Related Concept Videos

Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Receptor-Mediated Endocytosis01:20

Receptor-Mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis01:16

Endocytosis

Eukaryotic cells acquire nutrients for growth and proliferation. Nutrients and other molecules that require degradation are internalized from the extracellular space by a process called endocytosis. The term ‘endocytosis' was first coined by Christian de Duve in 1963.
Endocytosis always begins with the plasma membrane enclosing an incoming molecule to form a transport vesicle which, in some cases, can be coated with a protein called ‘clathrin.' Endocytosed material is either sorted through...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

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Related Experiment Video

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Viral Nanoparticles for In vivo Tumor Imaging
14:04

Viral Nanoparticles for In vivo Tumor Imaging

Published on: November 16, 2012

Endocytic uptake pathways utilized by CPMV nanoparticles.

Emily M Plummer1, Marianne Manchester

  • 1Skaggs School of Pharmacy and Pharmaceutical Sciences University of California, San Diego, La Jolla, California 92093, United States.

Molecular Pharmaceutics
|August 22, 2012
PubMed
Summary
This summary is machine-generated.

Cowpea mosaic virus (CPMV) nanoparticles enter cells via clathrin-independent pathways, utilizing caveolar and macropinocytosis routes. Understanding this viral nanoparticle trafficking enhances its potential for targeted drug delivery.

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Area of Science:

  • Nanotechnology
  • Virology
  • Cell Biology

Background:

  • Cowpea mosaic virus (CPMV) serves as a versatile nanoparticle platform for biomedical applications.
  • Effective use in vaccine development, imaging, and drug delivery requires understanding CPMV cell entry and trafficking.

Purpose of the Study:

  • To elucidate the specific endocytic pathways and intracellular trafficking mechanisms of CPMV in mammalian cells.
  • To identify how CPMV navigates cellular compartments after internalization.

Main Methods:

  • Utilized pharmacologic inhibition of cellular pathways.
  • Performed colocalization studies with specific endocytic vesicle markers.
  • Tracked CPMV particle movement within cells.

Main Results:

  • CPMV internalization is independent of clathrin-mediated endocytosis.
  • CPMV utilizes a combination of caveolar and macropinocytosis pathways for cellular entry.
  • CPMV particles traffic through Rab5-positive early endosomes to a lysosomal compartment.

Conclusions:

  • CPMV employs distinct endocytic mechanisms for cell entry.
  • The identified trafficking route provides insights for optimizing CPMV-based delivery systems.
  • Further development of intracellular drug-delivery strategies using CPMV is facilitated.