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Related Experiment Video

Updated: May 19, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Measuring antibody-antigen binding kinetics using surface plasmon resonance.

Stephen Hearty1, Paul Leonard, Richard O'Kennedy

  • 1Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2012
PubMed
Summary
This summary is machine-generated.

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Surface Plasmon Resonance (SPR) technology enables real-time, label-free monitoring of protein-protein interactions, ideal for screening antibody candidates. This method efficiently ranks antibodies based on binding kinetics for optimal selection.

Area of Science:

  • Biophysical techniques
  • Immunology
  • Biochemistry

Background:

  • Surface Plasmon Resonance (SPR) is a label-free technology widely used for studying molecular interactions.
  • It is particularly valuable for characterizing antibody-antigen interactions in real-time.
  • SPR is suitable for screening crude, unpurified antibody samples common in early selection stages.

Purpose of the Study:

  • To outline generic SPR-based configurations and methodologies for antibody screening.
  • To expedite the data-rich ranking of candidate antibody panels.
  • To ensure reliable identification of antibodies with optimal kinetic binding characteristics.

Main Methods:

  • Utilizing Surface Plasmon Resonance (SPR) for real-time, label-free detection of binding events.

More Related Videos

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Related Experiment Videos

Last Updated: May 19, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

  • Implementing generic screening configurations to assess antibody-antigen interactions.
  • Analyzing kinetic parameters, including association and dissociation rate constants.

    • Main Results:

    • SPR provides both concentration and affinity data for molecular interactions.
    • The technique allows for the resolution of discrete kinetic parameters (kon, koff).
    • SPR facilitates efficient screening and ranking of antibody candidates based on binding kinetics.

    Conclusions:

    • SPR is an effective technology for label-free, real-time monitoring of antibody-antigen interactions.
    • Standardized SPR methods can accelerate the selection and characterization of high-affinity antibodies.
    • This approach ensures the identification of antibodies with desirable kinetic profiles for therapeutic development.