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Related Experiment Video

Updated: May 19, 2026

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Quantitative chromosome conformation capture.

Raffaella Nativio¹, Yoko Ito, Adele Murrell

¹Department of Oncology, CRUK Cambridge Research Institute, University of Cambridge, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)

|August 22, 2012

Summary

This summary is machine-generated.

Chromatin folds into specific structures within the nucleus, enabling interactions between distant DNA elements like enhancers and promoters. The Chromosome Conformation Capture (3C) assay detects these crucial chromatin loops.

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Last Updated: May 19, 2026

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

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Published on: May 12, 2023

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

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Capturing Chromosome Conformation Across Length Scales

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Published on: January 20, 2023

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

Chromatin organization within the nucleus is highly specific, not random.
Higher-order chromatin structure facilitates interactions between distant genomic elements, such as enhancers and promoters.
These interactions are crucial for gene regulation.

Purpose of the Study:

To describe the Chromosome Conformation Capture (3C) methodology.
To highlight the power and limitations of the 3C assay.
To outline essential controls and normalization steps for accurate 3C analysis.

Main Methods:

Detailed description of the Chromosome Conformation Capture (3C) technique.
Explanation of how 3C detects chromatin looping.
Assessment of the probability of interaction between distant genomic elements.

Main Results:

The 3C assay is a powerful tool for detecting chromatin looping.
3C can quantify the interaction frequency between specific genomic loci.
The study details necessary controls and normalization for reliable results.

Conclusions:

The 3C assay provides valuable insights into nuclear chromatin organization.
Understanding chromatin conformation is key to deciphering gene regulation.
Accurate application of 3C methodology is essential for robust conclusions.