Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing coffee quality under humid subtropical conditions through ecological modulation of wet fermentation with an indigenous yeast starter.

International journal of food microbiology·2026
Same author

Biomass and precision fermentation inputs for cultivated meat: Advances, sustainability, food safety, and techno-economic perspectives.

Food research international (Ottawa, Ont.)·2026
Same author

An update on the application of alternative methods for evaluating antivenom potency.

Toxicon : official journal of the International Society on Toxinology·2026
Same author

Extreme Glycosyl Hydrolases: strategic biological agents for the circular economy.

Preparative biochemistry & biotechnology·2026
Same author

Unlocking the pharmaceutical potential of Kombucha: production, regulatory challenges and patents landscape.

Food science and biotechnology·2026
Same author

Soy-Derived Agroindustrial Byproducts as Sustainable Substrates for Biohydrogen Production via Dark Fermentation.

ACS omega·2026

Related Experiment Video

Updated: May 19, 2026

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
13:47

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Published on: May 19, 2020

Glycerol-based sterilization bioindicator system from Bacillus atrophaeus: development, performance evaluation, and

Sandra R B R Sella1, Patricia Milla Gouvea, Vanessa F Gomes

  • 1Production and Research Centre of Immunobiological Products, Parana State Department of Health, Av. São Roque, 716, Piraquara, PR, 83302-200, Brazil. sella.sandra@gmail.com

Applied Microbiology and Biotechnology
|August 23, 2012
PubMed
Summary

This study introduces a novel, low-cost sterilization biological indicator system (BIS) using glycerol, a biodiesel byproduct. The developed system effectively detects sterilization failures, offering a sustainable and economical solution.

More Related Videos

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
10:03

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Published on: November 30, 2017

Related Experiment Videos

Last Updated: May 19, 2026

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
13:47

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Published on: May 19, 2020

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
10:03

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Published on: November 30, 2017

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Microbiology

Background:

  • Glycerol, a biodiesel byproduct, presents environmental and economic challenges due to excess production.
  • Developing value-added applications for glycerol is crucial for sustainable industrial practices.

Purpose of the Study:

  • To investigate the use of glycerol as a primary substrate for producing a low-cost sterilization biological indicator system (BIS).
  • To optimize the BIS production process and evaluate its performance against regulatory standards.

Main Methods:

  • A sequential experimental design was employed for product development and optimization.
  • Solid-state fermentation was used for Bacillus atrophaeus spores production.
  • Performance was evaluated against dry-heat and ethylene oxide sterilization methods.

Main Results:

  • The optimized recovery medium facilitated heat-damaged spore germination, yielding a D-value of 6.6 ± 0.1 min at 160°C.
  • Bacillus atrophaeus spores production reached 2.3 ± 1.2 × 10^8 CFU/g dry matter within 48 hours.
  • Non-sterile conditions were visually identified within 24 hours, and the BIS met regulatory requirements.

Conclusions:

  • This research presents the first sterilization BIS produced using glycerol as the sole carbon source.
  • The glycerol-based BIS offers significant cost reductions and a profitable avenue for biodiesel byproduct utilization.
  • The developed system demonstrates compliance with sterilization validation standards.