Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Randomized Controlled Clinical Trial of Allogeneic Mesenchymal Stromal Cell Infusion in Diabetic Kidney Disease.

Kidney international reports·2026

Same author

The role of endothelial colony forming cells in the treatment of limb ischaemia: challenges in clinical translation.

Stem cells translational medicine·2026

Same author

Compassion-focused group therapy improves depression, emotional eating, self-criticism and shame in people living with severe obesity: A single-centre, examiner-blind randomized controlled trial.

PloS one·2026

Same author

When Antibiotics Leave a Mark: Minocycline-Induced Hyperpigmentation.

Clinical case reports·2026

Same author

Survival Outcomes of BCG Only, BCG Plus EMDA-MMC or Upfront Radical Cystectomy in High-Risk Non-Muscle Invasive Bladder Cancers (NMIBCs): A Multicentre, International, Collaborative Study from Tertiary Referral Institutions.

Cancers·2026

Same author

ATRA treatment in the emergency department is associated with reduced early mortality in acute promyelocytic leukemia-a real-world multicenter retrospective cohort analysis.

Leukemia & lymphoma·2026

Search research articles

Related Experiment Video

Updated: May 19, 2026

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Published on: April 4, 2018

Isolation of circulating angiogenic cells.

Erin E Vaughan¹, Timothy O'Brien

¹Regenerative Medicine Institute, University College Hospital, National University of Ireland, Galway, Ireland.

Methods in Molecular Biology (Clifton, N.J.)

|August 24, 2012

Summary

Endothelial progenitor cells (EPCs) are crucial for blood vessel formation. This study details the isolation and identification of circulating angiogenic cells, a distinct subtype of EPCs involved in vascular repair.

Area of Science:

Cardiovascular Biology
Cell Biology
Regenerative Medicine

Background:

Endothelial progenitor cells (EPCs), identified in 1997, were initially thought to be key players in angiogenesis and vasculogenesis.
Circulating EPC levels are established biomarkers for cardiovascular risk, with lower levels correlating to increased adverse events.
Recent controversies challenge the definition and function of EPCs, necessitating clearer classification.

Purpose of the Study:

To address the ongoing debate regarding the definition and function of EPCs.
To classify EPCs into two distinct groups: circulating angiogenic cells (CACs) and endothelial colony-forming cells (ECFCs).
To describe the methods for isolating and identifying CACs.

Main Methods:

Isolation of circulating angiogenic cells from blood samples.

More Related Videos

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

Related Experiment Videos

Last Updated: May 19, 2026

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Published on: April 4, 2018

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

Identification and characterization of the isolated cell population.

Analysis of the cellular mechanisms underlying angiogenic effects.

Main Results:

Distinction established between CACs (monocyte-enriched, paracrine signaling) and ECFCs (true EPCs, vessel incorporation).
Successful isolation and identification of circulating angiogenic cells.
Demonstration of CACs' role in promoting angiogenesis via paracrine mechanisms.

Conclusions:

Circulating angiogenic cells represent a distinct cell population with specific roles in vascular repair.
Clarifying EPC subtypes is essential for understanding their contribution to cardiovascular health and disease.
The described methods facilitate further research into CACs and their therapeutic potential.