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A three-way junction aptasensor for lysozyme detection.

Yunfeng Xia1, Siwen Gan, Qinghao Xu

  • 1Life and Environmental Science College, Shanghai Normal University, Guilin Road 100, Shanghai 200234, PR China.

Biosensors & Bioelectronics
|August 28, 2012
PubMed
Summary

A novel aptasensor for lysozyme detection utilizes a three-way junction DNA structure. This biosensor offers sensitive and specific lysozyme detection by monitoring changes in electron transfer signals.

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Area of Science:

  • Biotechnology
  • Biosensor Development
  • Molecular Diagnostics

Background:

  • Lysozyme is a key biomarker in various physiological and pathological processes.
  • Accurate lysozyme detection is crucial for disease diagnosis and monitoring.
  • Existing detection methods often lack specificity or require complex procedures.

Purpose of the Study:

  • To develop a highly sensitive and specific aptasensor for lysozyme detection.
  • To utilize a three-way junction (TWJ) DNA structure for enhanced sensing capabilities.
  • To establish a simple and efficient fabrication and regeneration process for the biosensor.

Main Methods:

  • Fabrication of a ferrocene (Fc)-tagged complementary DNA (cDNA) partially hybridized with an anti-lysozyme aptamer.
  • Formation of a TWJ structure with coaxial helix stacking enabling electron transfer (eT).
  • Monitoring the change in redox signal due to lysozyme binding-induced conformational changes blocking eT.

Main Results:

  • The TWJ aptasensor demonstrated signal attenuation upon lysozyme binding, indicating target presence.
  • Achieved a low detection limit of 0.2 nM with a wide dynamic range up to 100 nM.
  • Exhibited superior specificity and simpler regeneration compared to electrochemical impedance spectroscopy (EIS)-based methods.

Conclusions:

  • The developed TWJ aptasensor provides a sensitive, specific, and efficient platform for lysozyme detection.
  • The single-step fabrication and simplified regeneration offer practical advantages for clinical applications.
  • This aptasensor represents a promising advancement in electrochemical biosensing for biomarker quantification.