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Mutational Status of FGFR3 in Oral Squamous Cell Carcinoma
P Motahhary1, F Baghaie, S Mamishi
1Assistant Professor, Dental Research Center of Tehran University of Medical Sciences, Tehran, Iran.
Objective:
Head and neck squamous cell carcinoma, including oral squamous cell carcinoma (OSCC) is the sixth most common cancer in the human population. Despite significant efforts committed in treatment of OSCC the overall survival rate of OSCC has not improved significantly. Activating mutations in the fibroblast growth factor receptor 3 (FGFR3) genes are responsible for some human cancers, including bladder and cervical carcinoma. Despite a high frequency in some benign skin disorders, FGFR3 mutations have not been reported in cutaneous malignancies. Therefore, FGFR3 gene may play a role in epithelial biology and mutations of FGFR3 gene may contribute to the development of OSCC.
Materials And Methods:
In this cross-sectional study, DNA was extracted and purified from snap frozen tissue biopsy sections of 20 OSCC cases. Exons 7 and 15 were amplified by polymerase chain reaction (PCR) and sequenced in both directions.
Results:
In three cases silent mutations were identified in exon 7 (882 T to C) which may be introduced as Single Nucleotide Polymorphism (SNP) and no mutation was identified in exon 15.
Conclusion:
FGFR3 gene mutation in exon 7 and 15 has no significant role in the development and progression of OSCC. Analyzing other exons or considering other advanced gene mutation assessment techniques may clarify the role of this receptor mutation in OSCC pathogenesis.
Insights
Fibroblast growth factor receptor 3 (FGFR3) gene mutations in exons 7 and 15 do not significantly contribute to oral squamous cell carcinoma (OSCC) development. Further research into other FGFR3 exons is needed to clarify its role in OSCC pathogenesis.
Area of Science:
- Oncology
- Genetics
- Molecular Biology
Background:
- Oral squamous cell carcinoma (OSCC) is a prevalent head and neck cancer with stagnant survival rates.
- Activating mutations in fibroblast growth factor receptor 3 (FGFR3) are implicated in various cancers.
- The role of FGFR3 mutations in cutaneous malignancies and OSCC pathogenesis remains largely unexplored.
Purpose of the Study:
- To investigate the potential role of fibroblast growth factor receptor 3 (FGFR3) gene mutations in the development of oral squamous cell carcinoma (OSCC).
- To analyze specific exons (7 and 15) of the FGFR3 gene for mutations in OSCC tissue samples.
Main Methods:
- DNA was extracted from 20 OSCC tissue biopsy samples.
- Exons 7 and 15 of the FGFR3 gene were amplified using polymerase chain reaction (PCR).
- PCR products were sequenced in both directions to identify mutations.
Main Results:
- Silent mutations (882 T to C), potentially Single Nucleotide Polymorphisms (SNPs), were detected in exon 7 in three OSCC cases.
- No mutations were identified in exon 15 of the FGFR3 gene across the studied OSCC samples.
Conclusions:
- FGFR3 gene mutations in exons 7 and 15 do not appear to play a significant role in the development or progression of OSCC.
- Further investigation analyzing other FGFR3 exons or employing advanced gene mutation assessment techniques is recommended to fully elucidate the receptor's role in OSCC pathogenesis.
