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Relation between expression pattern of wild-type p53 and multidrug resistance proteins in human nephroblastomas

Ingrid Hodorová1, Silvia Rybárová, Janka Vecanová

  • 1Department of Anatomy, P.J. Šafárik University, Faculty of Medicine, Šrobárova 2, SK-04180 Košice, Slovak Republic.

Acta Histochemica
|August 29, 2012
PubMed

Insights

Multidrug resistance (MDR) proteins P-glycoprotein (Pgp/MDR1) and multidrug-resistant related protein (MRP1) were evaluated in pediatric Wilms' tumors. While MRP1 expression was significantly different from wild-type p53, no correlation was found between MDR proteins and p53.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Multidrug resistance (MDR) is a significant challenge in cancer therapy, often mediated by P-glycoprotein (Pgp/MDR1) and multidrug-resistant related protein (MRP1).
  • p53 tumor suppressor gene alterations may also contribute to therapeutic failure in various cancers, including Wilms' tumor.

Purpose of the Study:

  • To investigate the expression of Pgp/MDR1, MRP1, and wild-type p53 in pediatric Wilms' tumor samples.
  • To determine potential correlations between these proteins and their implications for treatment strategies.

Main Methods:

  • Indirect immunohistochemistry was employed to detect Pgp/MDR1, MRP1, and wild-type p53 expression.
  • 25 Wilms' tumor tissue samples from children were analyzed.

Main Results:

  • Positive expression for Pgp/MDR1 was found in 24% of samples, MRP1 in 48%, and wild-type p53 in 8%.
  • A statistically significant difference was observed between p53 and MRP1 expression (p<0.01), but not between p53 and Pgp/MDR1 (p>0.05).
  • No correlation was identified between the expression of MDR proteins and wild-type p53.

Conclusions:

  • The expression of MDR proteins and wild-type p53 varies in Wilms' tumors.
  • Immunohistochemical analysis of these markers at diagnosis could potentially guide the selection of chemotherapeutics for improved patient outcomes.

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