Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 19, 2026

Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings
07:37

Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

Published on: August 5, 2021

Open-cell recording of action potentials using active electrode arrays.

Dries Braeken1, Danny Jans, Roeland Huys

  • 1Bio-Nano Electronics, Imec, Kapeldreef 75, Leuven, 3001, Belgium. dries.braeken@imec.be

Lab on a Chip
|August 30, 2012
PubMed
Summary

This study introduces a novel electrode array for intracellular recordings, enabling long-term, high-resolution cellular electrophysiology. The minimally invasive technique allows for detailed investigation of cellular communication and drug effects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interactions between Notch and MAPK signaling in neurovascular development: Implications for advanced disease modeling and tissue engineering.

Neural regeneration research·2026
Same author

Continuous, Label-Free Phenotyping of Single Cells Based on Antibody Interaction Profiling in Microfluidic Channels.

Analytical chemistry·2025
Same author

Impedance mapping with high-density microelectrode array chips reveals dynamic heterogeneity of in vitro epithelial barriers.

Scientific reports·2025
Same author

Charge Trapping and Emission during Bias Temperature Stressing of Schottky Gate GaN-on-Silicon HEMT Structures Targeting RF/mm Wave Power Amplifiers.

Micromachines·2024
Same author

SUPERCELLS: a novel microfluidic reactor architecture for ultra-fast sequential delivery of chemical reagents.

Lab on a chip·2024
Same author

Electrophysiological properties of dorsal root ganglion neurons cultured on 3D silicon micro-pillar substrates.

Journal of neuroscience methods·2024

Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Cellular Electrophysiology

Background:

  • Advanced tools are needed for studying complex cellular communication.
  • Current electrode arrays primarily record extracellular potentials, limiting detailed analysis.
  • There is a need for high-throughput, minimally invasive intracellular recording methods.

Purpose of the Study:

  • To develop a novel electrode array for high-density intracellular action potential recording.
  • To enable minimally invasive, long-term intracellular measurements.
  • To apply the technique for investigating drug effects on cardiac electrical activity.

Main Methods:

  • Fabrication of a 16,384-electrode array using titanium nitride (TiN) on silicon.
  • Utilizing local membrane electroporation via micrometer-sized electrodes for transient intracellular access.

More Related Videos

A Multi-Electrode Array Platform for Modeling Epilepsy Using Human Pluripotent Stem Cell-Derived Brain Assembloids
06:30

A Multi-Electrode Array Platform for Modeling Epilepsy Using Human Pluripotent Stem Cell-Derived Brain Assembloids

Published on: September 27, 2024

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
11:28

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

Published on: February 9, 2022

Related Experiment Videos

Last Updated: May 19, 2026

Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings
07:37

Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

Published on: August 5, 2021

A Multi-Electrode Array Platform for Modeling Epilepsy Using Human Pluripotent Stem Cell-Derived Brain Assembloids
06:30

A Multi-Electrode Array Platform for Modeling Epilepsy Using Human Pluripotent Stem Cell-Derived Brain Assembloids

Published on: September 27, 2024

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
11:28

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

Published on: February 9, 2022

  • Implementing adaptable electroporation protocols to control access duration.
  • Performing consecutive intracellular recordings from the same cell over multiple days.

    • Main Results:

    • Successful intracellular action potential recording at each electrode site.
    • Demonstration of transient and tunable intracellular access.
    • Confirmation of minimally invasive properties for short- and long-term recordings.
    • Application of the method to study ion channel blocker effects on cardiac cells.

    Conclusions:

    • The developed TiN electrode array enables massively parallel, long-term intracellular recordings.
    • This technique offers a significant advancement for fundamental electrophysiology research.
    • The method is suitable for high-throughput drug screening and cellular communication studies.