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Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

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Stuttering Min oscillations within E. coli bacteria: a stochastic polymerization model.

Supratim Sengupta¹, Julien Derr, Anirban Sain

¹Centre for Computational Biology and Bioinformatics, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. supratim.sen@iiserkol.ac.in

Physical Biology

|August 31, 2012

Summary

We developed a 3D model to study bacterial Min protein oscillations in E. coli. Our findings reveal factors influencing oscillation stuttering, offering insights into bacterial cell division regulation.

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Area of Science:

Cell Biology
Biophysics
Computational Biology

Background:

The Min system in Escherichia coli regulates cell division by forming dynamic protein filaments.
Subcellular oscillations of Min proteins are crucial for proper placement of the division septum.
Min oscillation stuttering is an experimental phenomenon that requires mechanistic explanation.

Purpose of the Study:

To develop and utilize a 3D off-lattice stochastic polymerization model.
To investigate the factors affecting Min oscillation stuttering in E. coli.
To understand the relationship between polymerization dynamics and oscillation characteristics.

Main Methods:

Developed a 3D off-lattice stochastic polymerization model.
Simulated Min protein dynamics, including polymerization, depolymerization, and interactions.
Analyzed oscillation patterns, stuttering frequency, and filament properties.

Main Results:

Processivity of MinE rebinding, protection of filament tips, and filament fragmentation influence stuttering.
Each factor individually reduces stuttering, speeds up oscillations, and shortens MinD filaments.
Filament fragmentation alone can lead to fast, infrequent stuttering, but excessive fragmentation disrupts oscillations.

Conclusions:

Fast, stutter-free oscillations require a combination of processivity and tip protection.
Short MinD filaments correlate with infrequent stuttering in standard Min oscillations.
Increased stuttering frequency may serve as a diagnostic for observable MinD polymerization.