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Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Protein Complexes with Interchangeable Parts

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Assembly of Signaling Complexes

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Related Experiment Video

Updated: May 19, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

14-3-3 phosphoprotein interaction networks - does isoform diversity present functional interaction specification?

Anna-Lisa Paul¹, Fiona C Denison, Eric R Schultz

¹Program in Plant Molecular and Cellular Biology, Horticultural Science Department, University of Florida Gainesville, FL, USA.

Frontiers in Plant Science

|August 31, 2012

Summary

Arabidopsis 14-3-3 proteins are key signaling nodes. This study provides evidence that sequence diversity among these proteins indicates functional specificity, impacting signal transduction research.

Keywords:

14-3-3 isoform specificity Arabidopsis GRF plant subcellular localization

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

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Area of Science:

Plant molecular biology
Proteomics
Signal transduction

Background:

14-3-3 proteins are crucial phosphoprotein interactors in plants.
They form a central hub in the Arabidopsis Interactome Map, mediating numerous signaling pathways.
Evidence suggests potential isoform-specific functions, but also functional redundancy among 14-3-3 family members.

Purpose of the Study:

To investigate whether sequence diversity in Arabidopsis 14-3-3 protein isoforms correlates with functional diversity at biochemical or cellular levels.
To resolve the debate on functional specificity versus redundancy of 14-3-3 isoforms.

Main Methods:

Review and discussion of existing observations on 14-3-3 protein function.
Presentation of contrastable observations to support the hypothesis of functional diversity.
Development of a model to explain 14-3-3 isoform specificity.

Main Results:

The study presents a model strongly suggesting functional specificities among Arabidopsis 14-3-3 isoforms.
Sequence diversity is indicative of distinct biochemical or cellular roles.

Conclusions:

Functional specificities exist within the Arabidopsis 14-3-3 protein family.
14-3-3 diversity and specificity should be considered in signal transduction research and experimental design.