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Updated: May 19, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
VDAC3 regulates centriole assembly by targeting Mps1 to centrosomes
Shubhra Majumder1, Mark Slabodnick, Amanda Pike
1Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
Abstract:
Centrioles are duplicated during S-phase to generate the two centrosomes that serve as mitotic spindle poles during mitosis. The centrosomal pool of the Mps1 kinase is important for centriole assembly, but how Mps1 is delivered to centrosomes is unknown. Here we have identified a centrosome localization domain within Mps1 and identified the mitochondrial porin VDAC3 as a protein that binds to this region of Mps1. Moreover, we show that VDAC3 is present at the mother centriole and modulates centriole assembly by recruiting Mps1 to centrosomes.
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