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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
The cold adaptability of microorganisms with different carbon source in activated sludge treating synthetical
Chuan Niu1, Jinju Geng, Hongqiang Ren
1State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China.
Abstract:
The cold adaptability of microorganisms with different carbon source under 5°C was studied in activated sludge for treating synthetical wastewater. Phospholipid fatty acid (PLFA) analysis indicated contents of unsaturated fatty acids in cell membrane at 5°C were 13.66% and 24.96% higher for glucose and sodium acetate source than that at 25°C. PLFA biomarkers showed more Gram-negative bacteria enriched than Gram-positive bacteria in low-temperature activated sludge. The Shannon-Wiener diversity analysis demonstrated glucose fed reactor in low temperature had lower PLFA diversity index (1.21-1.30) than that at 25°C and sodium acetate source was reverse (1.08-0.69). The 16S rRNA analysis manifested certain microbes were considerably suitable for existence under cold environment, most of which belong to Gram-negative bacteria.
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