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Updated: May 19, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
Published on: November 2, 2018
BASHD-J-resolved-COSY: a new method for measuring proton-proton spin coupling constants of multiplet signals
Kazuo Furihata1, Mitsuru Tashiro
1Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Tokyo 113-8657, Japan. afuriha@mail.ecc.u-tokyo.ac.jp
Abstract:
An effective pulse sequence for measuring H-H coupling constants, named BASHD-J-resolved-COSY, has been developed. In the spin systems such as -CH(A)-CH(B)(CH(3))-CH(C)-, a methine proton H(B) splits into a multiplet owing to several vicinal couplings, resulting in attenuation of its cross-peak intensity. Therefore, the measurements of (3)J(H-H) with respect to H(B) are generally difficult in the E-COSY-type experiments. With the aim of accurate measurements of (3)J(H-H) in such a spin system, we have developed a new pulse sequence, which selectively decouples the secondary methyl group. The proposed pulse sequence provides the simplified cross-peak patterns, which are suitable for reliable measurements of (3)J(H-H) in a complicated natural product.
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