[Bioaugmented removal of pyridine and the microbial community dynamic analysis]

The bioaugmented removal of pyridine was investigated through introducing immobilized Paracoccus sp. strain KT-5 capable of degrading pyridine into the lab-scale sequencing batch reactor (SBR) inoculated with activated sludge. The terminal restriction fragment length polymorphisms (T-RFLP) was used to analyzed the microbial community dynamics of two reactors during the whole operation process. The experimental results indicated that the introduction of immobilized strain KT-5 into the SBR could speed up the start-up of reactor, compared to the non-bioaugmented SBR. When the initial concentration of pyridine varied from 195.6 mg x L(-1) to 586.8 mg x L(-1), the bioaugmented effect was not significant; however, when the initial concentration of pyridine was 782.4-2934 mg x L(-1), the bioaugmentation role in pyridine degradation was obvious. The analysis of T-RFLP indicated that the introduced immobilized strain KT-5, as a dominant strain, always existed in both free and immobilized biomass of the bioaugmented SBR.