Related Experiment Video
Updated: May 19, 2026
11:00
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
Rachycentron canadum (cobia) lectin promoted mitogenic response in mice BALB/c splenocytes
M C Coriolano1, C M L de Melo, A J G Santos
1Departamento de Bioquímica, Laboratório de Glicoproteínas da Universidade Federal de Pernambuco-UFPE, Recife, Brazil.
Scandinavian Journal of Immunology
|September 6, 2012
Summary
Cobia fish lectin (RcaL) effectively stimulates mouse splenocyte proliferation and cytokine production, acting as a potent mitogenic agent for immune cell research. This lectin shows promise for immunostimulatory assays.
Area of Science:
- Immunology
- Biochemistry
- Marine Biology
Background:
- Mitogenic lectins are crucial for studying lymphocyte activation and immune cell proliferation.
- Rachycentron canadum lectin (RcaL) was identified and purified from cobia fish serum.
Purpose of the Study:
- To assess the in vitro effects of RcaL on mouse splenocyte proliferation and cytokine production.
- To compare RcaL's efficacy against the positive control, Canavalia ensiformis lectin (Con A).
Main Methods:
- In vitro stimulation of mouse splenocytes with RcaL.
- Measurement of splenocyte proliferation index.
- Quantification of Interleukin-2 (IL-2) and Interleukin-6 (IL-6) production.
- Assessment of cell viability and apoptosis.
Main Results:
- RcaL significantly increased splenocyte proliferation compared to control cells.
- RcaL induced higher levels of IL-2 and IL-6 production.
- Cell viability remained high (90%) with RcaL treatment, despite significant late apoptosis induction at 24 and 48 hours.
Conclusions:
- RcaL demonstrates potent mitogenic activity, stimulating immune cell proliferation and cytokine release.
- RcaL can be utilized as an effective immunostimulatory agent in experimental assays.
- Further research into RcaL's immunomodulatory mechanisms is warranted.
