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Updated: May 19, 2026
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Microsatellite markers developed for Utricularia reniformis (Lentibulariaceae)
Débora Clivati1, Matthew Aaron Gitzendanner, Alexandre Wagner Silva Hilsdorf
1Universidade de Mogi das Cruzes, Núcleo Integrado de Biotecnologia, Av. Cândido Xavier de Almeida e Souza 200, Mogi das Cruzes, São Paulo 08780-911, Brazil. deboraclivati@yahoo.com.br
Premise Of The Study:
Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.
Methods And Results:
The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplification was successful in three other Utricularia species and one Pinguicula species.
Conclusions:
Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula.
