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Isoorientin reverts TNF-α-induced insulin resistance in adipocytes activating the insulin signaling pathway

Angel Josabad Alonso-Castro1, Rocio Zapata-Bustos, Guadalupe Gómez-Espinoza

  • 1Instituto Potosino de Investigación Científica y Tecnológica, Molecular Biology Division, San Luis Potosí, México.

Endocrinology
|September 6, 2012
PubMed

Insights

Isoorientin (ISO) activates the insulin signaling pathway in fat cells, enhancing glucose uptake. This plant flavonoid also reverses insulin resistance induced by TNF-α, offering potential antidiabetic benefits.

Area of Science:

  • Biochemistry
  • Metabolic Research
  • Pharmacology

Background:

  • Isoorientin (ISO), a plant C-glycosylflavonoid, is suggested to possess antidiabetic properties.
  • The precise mechanisms underlying ISO's potential antidiabetic effects remain largely unelucidated.

Purpose of the Study:

  • To investigate the antidiabetic mechanisms of Isoorientin (ISO) in adipocytes.
  • To determine if ISO influences glucose uptake and insulin signaling pathways.

Main Methods:

  • Assessed 2-NBDG uptake in murine 3T3-F442A and human adipocytes treated with ISO.
  • Evaluated ISO's effects on insulin signaling pathway components and gene expression.
  • Utilized TNF-α to induce insulin resistance in adipocytes for comparative analysis.

Main Results:

  • ISO significantly stimulated glucose uptake in both insulin-sensitive and insulin-resistant adipocytes.
  • ISO's glucose uptake induction was dependent on the insulin signaling pathway.
  • ISO treatment promoted proper phosphorylation of insulin signaling proteins and stimulated relevant gene transcription.

Conclusions:

  • Isoorientin (ISO) exerts antidiabetic effects by activating the insulin signaling pathway in adipocytes.
  • ISO effectively reverses TNF-α-induced insulin resistance by modulating key signaling proteins and gene expression.

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