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Updated: May 18, 2026
Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration
Published on: June 7, 2014
Protective effect of infliximab on ischemia/reperfusion-induced damage in rat kidney
Cemal Tasdemir1, Seda Tasdemir, Nigar Vardi
1Department of Urology, Inonu University, Medical Faculty, Malatya, Turkey. tasdemrcemal@yahoo.com
Objective:
To investigate the protective effect of infliximab on ischemia-reperfusion (I/R) injury of the rat kidney.
Methods:
Twenty-eight male Wistar albino rats were divided into four groups: sham-operated, I/R, I/R with infliximab administered before ischemia [I/R + infliximab (bi)], and I/R with infliximab administered before reperfusion [I/R + infliximab (br)]. After a right nephrectomy to produce damage, the left renal vessels were occluded for 60 min, followed by 24-h reperfusion in rats. Changes in the rat kidney were observed by measuring the tissue levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD) and by evaluating hematoxylin-eosin (H&E)-stained and periodic acid-Schiff (PAS) sections.
Results:
The MDA and MPO levels in the I/R group were significantly higher than in the other groups (p < 0.05), and the SOD and GSH levels in the I/R + infliximab (bi) and I/R + infliximab (br) groups were significantly higher than in the I/R group (p < 0.05). However, histological examination revealed that the I/R + infliximab (bi) group and the I/R + infliximab (br) group had significantly fewer tubular changes and interstitial inflammatory cell infiltration than the I/R group.
Conclusion:
These results show that infliximab may protect against I/R injury in the rat I/R model.
