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Updated: May 18, 2026
Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits
André Luís Alonso Domingos1, Sérgio Britto Garcia, José de Bessa Junior
1Department of Surgery, Division of Urology, Ribeirão Preto Medical School - University of Sao Paulo Ribeirão Preto, SP and Department of Experimental Surgery, Anhanguera-Uniderp University, Campo Grande, MS, Brazil.
Objective:
To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits.
Materials And Methods:
After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5µm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry.
Results:
No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001).
Conclusion:
The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process.
