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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structure characterization of a novel neutral polysaccharide isolated from Ganoderma lucidum fruiting bodies
Deng Pan1, Linqiang Wang, Congheng Chen
1State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
Abstract:
Ganoderma lucidum (G. lucidum) is a mushroom which has been used for health promotion for a long time in China. In the present work a neutral hetero-polysaccharide, named FYGL-1, was isolated from FYGL which was reported previously capable of antihyperglycemia in vivo for further detailed chemical structure investigation. The results of monosaccharide composition and GPC analysis indicated that FYGL-1 consisted of galactose, rhamnose and glucose in mole ratio of 1.00:1.15:3.22 with a molecular weight of 78kDa. The detailed structure of FYGL-1 was characterized by periodate oxidation, Smith degradation, methylation analysis, along with FT-IR, GC, GC-MS, 1D (1)H and (13)C NMR and 2D NMR (HSQC, COSY, NOESY and TOCSY). Based on the analysis of the results, the structure of the repeating unit of FYGL-1 was established as:
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