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Updated: May 18, 2026
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In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
Bioluminescence based in vivo screening technologies
1Molecular Functional Imaging Lab, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Sector 22, Kharghar, Navi Mumbai 410210, Maharashtra, India.
Current Opinion in Pharmacology
|September 8, 2012
Summary
Bioluminescence assays, using luciferase, offer sensitive and specific monitoring of cellular functions. These methods are valuable for drug screening and in vivo imaging in research.
Area of Science:
- Biochemistry
- Molecular Biology
- Biotechnology
Background:
- Bioluminescence is a natural light-producing event.
- Cell-based biosensors utilizing bioluminescence enable dynamic monitoring of cellular functions.
- Luciferase assays are highly sensitive and specific, especially when luciferase is not native to the organism.
Purpose of the Study:
- To review various bioluminescence screening strategies.
- To analyze the scope of these strategies in future drug screening.
- To highlight the utility of bioluminescence in molecular imaging.
Main Methods:
- Review of existing literature on bioluminescence screening methods.
- Analysis of genetic and chemical modifications in luciferase-substrate systems.
- Exploration of in vivo bioluminescence imaging (BLI) applications.
Main Results:
- Bioluminescent cell-based biosensors are effective for monitoring cellular processes.
- Modified luciferase-substrate systems enhance assay robustness and functionality.
- In vivo bioluminescence imaging (BLI) is a powerful tool for studying human cellular biology in animal models.
Conclusions:
- Bioluminescence screening strategies are versatile and valuable for drug discovery.
- Advancements in bioluminescence technology continue to expand its applications in biological research and drug development.
