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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Organisms00:53

Transgenic Organisms

Overview
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: May 18, 2026

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
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Tet-On binary systems for tissue-specific and inducible transgene expression.

Daniel R Buchholz1

  • 1Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA. buchhodr@ucmail.uc.edu

Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2012
PubMed
Summary

Researchers developed a Tet-On system for Xenopus, enabling doxycycline-inducible control of gene expression. This provides a flexible new tool for developmental biology studies using these transgenic lines.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Precise control over transgene expression is crucial for developmental biology research.
  • Existing Xenopus models lack widely available transgenic lines for inducible and tissue-specific gene expression, unlike mouse and zebrafish models.

Purpose of the Study:

  • To design and characterize a Tet-On binary system for inducible and tissue-specific transgene expression in Xenopus.
  • To create a resource of transgenic Xenopus lines for the scientific community.

Main Methods:

  • Developed two components for the Tet-On system: rtTA transgenic lines and Tet-Responsive Element (TRE) transgenic lines.
  • rtTA (reverse tetracycline-controlled transcriptional activator) is doxycycline-dependent and controlled by tissue-specific promoters.
  • TRE lines contain the gene of interest under the control of the TRE promoter.

Main Results:

  • The Tet-On system allows transgene expression to be turned on in the presence of doxycycline (Dox) and off in its absence.
  • Tissue specificity is dictated by the promoter driving rtTA expression.
  • Inducibility is achieved by administering Dox to the rearing water.

Conclusions:

  • The developed rtTA and TRE transgenic Xenopus lines offer a powerful and flexible system for tissue-specific, inducible control of gene expression.
  • These lines will serve as a valuable resource for advancing developmental biology research in Xenopus.