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Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
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Immunoisolation of protein complexes from Xenopus

Frank L Conlon1, Yana Miteva, Erin Kaltenbrun

  • 1Department of Genetics, University of North Carolina, Chapel Hill, NC, USA. frank_conlon@med.unc.edu

Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2012
PubMed
Summary

This chapter details immunoaffinity isolation methods for purifying protein complexes from Xenopus. Cryolysis and magnetic bead techniques preserve complexes and minimize yolk contamination for further proteomic analysis.

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