Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Broadband excitation of nuclei spin by refocused feedback.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2025
Same author

Exploring the Higher Order Structure and Conformational Transitions in Insulin Microcrystalline Biopharmaceuticals by Proton-Detected Solid-State Nuclear Magnetic Resonance at Natural Abundance.

Analytical chemistry·2024
Same author

An improved algorithm for design of broadband excitation, inversion, and mixing pulse sequences by iterative optimization of phases: TOPS-2.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2023
Same author

MODE: Multiply modulated frame technique, excitation and coherence transfer.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2023
Same author

Composite pulse combinations for chirp excitation.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2022
Same author

Chirp pulse sequences for broadband π rotation.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2021
Same journal

A data-driven modeling study on the accurate identification of Doppler-free saturated absorption spectra in diatomic tellurium (130Te2).

The Journal of chemical physics·2026
Same journal

Anharmonic phonons via quantum thermal bath simulations.

The Journal of chemical physics·2026
Same journal

Quantum simulation of alignment dependent differential cross sections in co-propagating molecular beams at cold collision energies.

The Journal of chemical physics·2026
Same journal

Non-additive ion effects on the coil-globule equilibrium of a generic polymer in aqueous salt solutions.

The Journal of chemical physics·2026
Same journal

Insights into the unexpected small reduction of the temperature of maximum density of water by lithium chloride addition.

The Journal of chemical physics·2026
Same journal

Optical frequency comb double-resonance spectroscopy of the 9030-9175 cm-1 states of ethylene.

The Journal of chemical physics·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Homonuclear decoupling for liquid-state NMR.

Van D M Koroleva1, Navin Khaneja

  • 1School of Engineering and Applied Sciences, Harvard University, 33 Oxford St., Cambridge, Massachusetts 02138, USA. vmdo@fas.harvard.edu

The Journal of Chemical Physics
|September 11, 2012
PubMed
Summary
This summary is machine-generated.

We developed new nuclear magnetic resonance (NMR) pulse sequences to effectively decouple homonuclear two-spin systems with weak scalar coupling. These methods create a field that overcomes the coupling, improving spectral resolution for NMR applications.

Related Experiment Videos

Last Updated: May 18, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Quantum Control
  • Spin Physics

Background:

  • Homonuclear two-spin systems with weak scalar coupling present challenges in NMR spectroscopy due to signal overlap.
  • Isotropic scalar coupling can obscure important chemical shift information, limiting spectral resolution.
  • Effective decoupling methods are crucial for simplifying complex NMR spectra.

Purpose of the Study:

  • To present a novel solution for decoupling homonuclear two-spin systems with weak isotropic scalar coupling.
  • To introduce non-selective pulse sequences that enable effective decoupling across a wide range of chemical shifts.
  • To provide methods that can enhance spectral resolution in NMR spectroscopy.

Main Methods:

  • Development of non-selective pulse sequences.
  • Creation of an effective magnetic field perpendicular to the coupling interaction.
  • Utilizing chemical shift differences to achieve decoupling.

Main Results:

  • The proposed pulse sequences generate an effective field with magnitude proportional to chemical shifts.
  • Effective decoupling is achieved when chemical shift differences exceed the coupling strength.
  • The methods effectively scale down chemical shifts, simplifying spectral analysis.

Conclusions:

  • The presented pulse sequences offer a viable solution for decoupling homonuclear two-spin systems.
  • These methods can be broadly applied in various nuclear magnetic resonance spectroscopy applications.
  • The technique enhances spectral resolution by overcoming limitations imposed by weak scalar couplings.