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Related Concept Videos

Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...

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In vivo 19F MRI for Cell Tracking
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In vivo 19F MRI for Cell Tracking

Published on: November 25, 2013

Labeling cells for in vivo tracking using (19)F MRI.

Mangala Srinivas1, Philipp Boehm-Sturm, Carl G Figdor

  • 1Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, 6500HB Nijmegen, The Netherlands. mangala.srinivas@gmail.com

Biomaterials
|September 11, 2012
PubMed
Summary

Fluorine-19 MRI (19F MRI) offers noninvasive, long-term cell tracking for immune cells and therapeutics. Overcoming cell loading challenges enables precise in vivo quantification and monitoring of cellular therapies.

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Area of Science:

  • Biomedical Imaging
  • Cellular Biology
  • Medical Diagnostics

Background:

  • Noninvasive in vivo cell tracking is essential for understanding mobile and transplanted cell functions, especially immune cells and cellular therapeutics.
  • Fluorine-19 MRI (19F MRI) provides advantages like longitudinal data acquisition, stable non-radioactive isotope use for long-term tracking, no confounding endogenous signal, and cell number quantification.
  • A significant challenge in 19F MRI for cell tracking is achieving sufficient signal through adequate cell loading, particularly for non-phagocytic cells like lymphocytes and stem cells.

Purpose of the Study:

  • To summarize current cell loading strategies for 19F MRI.
  • To review the sensitivity of in vivo cell imaging using 19F MRI.
  • To discuss image data processing for accurate cell number quantification.

Main Methods:

  • Development and application of various 19F cell labels (emulsions, particles, polymers, clinical agents).
  • Labeling and in vivo study of diverse cells (dendritic cells, lymphocytes, phagocytes) in animal models.
  • Testing of primary human cells, including dendritic cells for vaccine therapy, for clinical application.

Main Results:

  • Successful labeling and in vivo tracking of various cell types in models of autoimmune disease, inflammation, and transplant rejection.
  • Demonstration of 19F MRI's capability for longitudinal and quantitative cell tracking.
  • Advancements in cell loading strategies and imaging sensitivity are crucial for effective cell tracking.

Conclusions:

  • 19F MRI is a powerful, noninvasive tool for longitudinal and quantitative cell tracking in vivo.
  • Addressing cell loading challenges is key to maximizing the potential of 19F MRI for cellular therapeutics and research.
  • This technology holds significant promise for clinical applications, particularly in vaccine therapy and regenerative medicine.