Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cold-active hydrolases producing bacteria from two different sub-glacial Himalayan lakes.

Harmesh Sahay1, Bandamaravuri Kishore Babu, Surendra Singh

  • 1National Bureau of Agriculturally Important Microorganisms (NBAIM), Kusmaur, Mau, Uttar Pradesh, India. harmeshsahay@rediffmail.com

Journal of Basic Microbiology
|September 11, 2012
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electron-induced ionisation of the C₂F₆ molecule.

European journal of mass spectrometry (Chichester, England)·2026
Same author

Evaluating pXRF accuracy for predicting soil fertility: effects of moisture and soil properties.

Scientific reports·2026
Same author

Early Versus Conventional Discontinuation of Caffeine Therapy for Apnea of Prematurity: A Randomized Controlled Trial-The DECaf Trial.

Pediatric pulmonology·2026
Same author

Hydrophyte root microbiome: a novel reservoir of plant growth-promoting bacteria for enhancing lettuce (Lactuca sativa L.) growth and nutritional quality under soilless cultivation.

World journal of microbiology & biotechnology·2026
Same author

Integrated experimental and computational evaluation of novel benzopyran-2-one congeners as reactive oxygen species scavengers in inflammation.

Computers in biology and medicine·2026
Same author

Development of a synergistic tripartite consortium and hybrid growth media for enhanced methane oxidation and assessment of in vitro plant growth-promoting traits.

World journal of microbiology & biotechnology·2026

Himalayan lakes harbor diverse bacteria adapted to cold environments. These microbes yield unique cold-active enzymes with broad stability, offering significant biotechnological potential for various industrial applications.

Area of Science:

  • Microbiology
  • Biotechnology
  • Enzymology

Background:

  • Cold-adapted microorganisms possess unique enzymes with biotechnological applications.
  • Understanding bacterial diversity in extreme environments is crucial for discovering novel biocatalysts.

Purpose of the Study:

  • To analyze the diversity of culturable bacteria in Himalayan sub-glacial freshwater and brackish water lakes.
  • To identify and characterize cold-active hydrolases from these bacterial isolates.

Main Methods:

  • Bacterial isolation and cultivation.
  • SYBR green staining and amplified ribosomal DNA restriction analysis (ARDRA).
  • Phylogenetic analysis using 16S rRNA gene sequencing and screening for hydrolase activity.

Main Results:

Keywords:
16S rRNA geneCold-active hydrolasesHimalayan lakesPsychrophiles

Related Experiment Videos

  • 140 bacterial isolates were identified as psychrophiles, psychrotrophs, or psychrotolerant organisms.
  • Phylogenetic analysis placed isolates within Firmicutes, Proteobacteria, and Actinobacteria phyla.
  • 16 isolates exhibited significant cold-active hydrolase (protease, amylase, xylanase, cellulase) activity with broad thermal and pH stability.

Conclusions:

  • Himalayan freshwater and brackish lakes host distinct bacterial communities.
  • These bacteria are a valuable source of extracellular hydrolases with wide-ranging thermal stability for biotechnological use.