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Related Concept Videos

Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Decreased Body Temperature01:29

Decreased Body Temperature

A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by sustained extreme cold exposure, and severe...
Oxygen Transport in the Blood01:27

Oxygen Transport in the Blood

Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Coagulation01:09

Coagulation

The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.

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Related Experiment Video

Updated: May 18, 2026

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
08:22

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

Freezing does not decrease carbon monoxide-mediated hypercoagulation and hypofibrinolysis in human plasma.

Vance G Nielsen1, David T Hafner

  • 1Department of Anesthesiology, The University of Arizona College of Medicine, Tucson, Arizona 85724-5114, USA. vgnielsen@email.arizona.edu

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|September 12, 2012
PubMed
Summary
This summary is machine-generated.

Freezing plasma does not alter carbon monoxide

More Related Videos

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo
06:23

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

Published on: August 4, 2018

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Last Updated: May 18, 2026

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
08:22

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo
06:23

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

Published on: August 4, 2018

Area of Science:

  • Biochemistry
  • Hematology
  • Physiology

Background:

  • Carbon monoxide (CO) influences hemostasis by affecting coagulation and fibrinolysis.
  • Understanding CO's impact on heme-modulated molecules is crucial for hemostasis research.
  • Standardizing sample handling is essential for reliable results in multi-center studies.

Purpose of the Study:

  • To investigate the effect of freezing on carbon monoxide-mediated hemostatic changes in plasma.
  • To determine if a freeze-thaw cycle impacts CO's modulation of coagulation and fibrinolysis.
  • To establish reliable methods for collecting and transporting plasma samples in CO-related hemostasis studies.

Main Methods:

  • Plasma samples were exposed to varying concentrations of carbon monoxide (CO) using tricarbonyldichlororuthenium (II) dimer.
  • A portion of the CO-exposed plasma was immediately frozen at -80 °C.
  • Both unfrozen and subsequently thawed plasma samples underwent thrombelastographic analysis with and without tissue-type plasminogen activator (t-PA).

Main Results:

  • Freezing plasma did not significantly alter the enhancing effects of CO on coagulation.
  • The attenuation of fibrinolysis by CO remained unaffected by the freeze-thaw cycle.
  • CO-mediated hemostatic changes in plasma are robust and not compromised by standard freezing procedures.

Conclusions:

  • A freeze-thaw cycle does not interfere with carbon monoxide's effects on plasma coagulation and fibrinolysis.
  • These findings support the feasibility of collecting and transporting plasma samples on dry ice for CO-related hemostasis research.
  • The study validates a method for local batch processing of samples, ensuring consistency across collaborating centers.