Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature-derivative spectroscopy: a tool for protein dynamics.

J Berendzen1, D Braunstein

  • 1Department of Biophysics, University of Illinois, Urbana 61801.

Proceedings of the National Academy of Sciences of the United States of America
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diverse excess GABA modes drive autism and epilepsy-autism comorbidity.

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Ephaptic conduction molding memory engrams.

BMC biology·2025
Same author

Possible mechanism of schizophrenia origin by excess GABA and synaptic pruning.

IBRO neuroscience reports·2023
Same author

A possible new cardiac heterogeneity as an arrhythmogenic driver.

Scientific reports·2023
Same author

Percolation and tortuosity in heart-like cells.

Scientific reports·2021
Same author

Origin of post-ictal and post-anesthesia adverse effects and possibly of SUDEP.

Medical hypotheses·2021
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

A new temperature-derivative relaxation method reveals insights into carbon monoxide (CO) recombination with sperm whale myoglobin. This technique uncovers distributed activation enthalpies and kinetic hole burning, complementing existing methods.

Area of Science:

  • Biophysics
  • Chemical Kinetics

Background:

  • Myoglobin is a protein crucial for oxygen transport.
  • Understanding ligand recombination, like carbon monoxide (CO) to myoglobin, is vital for protein dynamics.
  • Previous studies often used isothermal methods to investigate these processes.

Purpose of the Study:

  • Introduce a novel temperature-derivative relaxation method.
  • Apply this method to study CO recombination kinetics in sperm whale myoglobin.
  • Investigate the geminate recombination process and ligand escape from the heme pocket.

Main Methods:

  • Developed a relaxation method measuring population change with respect to temperature.
  • Utilized infrared spectroscopy to monitor CO-stretch bands.
  • Analyzed data using gaussian enthalpy distributions.

Related Experiment Videos

Main Results:

  • Observed distributed activation enthalpies for CO recombination, varying across different infrared bands.
  • Identified transitions between bands corresponding to photolyzed ligands.
  • Detected kinetic hole burning phenomena.
  • Demonstrated that the temperature-derivative method complements isothermal techniques.
  • Explored CO recombination from outside the heme pocket.

Conclusions:

  • The temperature-derivative method provides a powerful tool for studying protein-ligand dynamics.
  • Distributed activation enthalpies are characteristic of CO recombination in myoglobin.
  • The technique offers complementary insights to established kinetic methods.