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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

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Introducing GUt low-density array (GULDA): a validated approach for qPCR-based intestinal microbial community

Anders Bergström¹, Tine R Licht, Andrea Wilcks

¹National Food Institute, Technical University of Denmark, Søborg, Denmark.

FEMS Microbiology Letters

|September 13, 2012

Summary

A new method, the GUt Low-Density Array (GULDA), enables rapid, comprehensive analysis of human gut microbiota alterations. This tool tracks 31 microbial targets, aiding health research.

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Area of Science:

Microbiology
Genomics
Human Health

Background:

Gut microbiota composition is crucial for host health and is influenced by factors like diet, age, and antibiotics.
Detecting gut microbiota alterations rapidly and comprehensively is essential for health research.

Purpose of the Study:

To develop and validate a high-throughput real-time quantitative PCR platform for simultaneous analysis of gut microbial targets.
To assess changes in gut microbiota composition over time in infants.

Main Methods:

Development of the GUt Low-Density Array (GULDA) platform.
Utilizing real-time quantitative PCR for simultaneous analysis of 31 microbial 16S rRNA gene targets.
Analysis of fecal samples from infants at 9 and 18 months of age.

Main Results:

GULDA successfully analyzed changes in the abundance of 31 microbial targets in fecal samples.
Significant increases in Clostridial cluster IV and Bifidobacterium bifidum were observed in infants from 9 to 18 months.
A decrease in Clostridium butyricum and a tendency for decrease in Enterobacteriaceae were noted.

Conclusions:

The GUt Low-Density Array (GULDA) is a validated tool for comprehensive gut microbiota analysis.
The study demonstrated significant shifts in infant gut microbiota composition during the first year of life.
GULDA facilitates rapid detection of microbial alterations relevant to host health.