Related Experiment Video
Updated: May 18, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
[Expression of PRUNE2 mRNA and its positive correlation with non-coding RNA PCA3 in leiomyosarcoma]
Ji-long Yang1, David Cogdell, James Eddy
1Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Hospital, China. jilongyang2003@yahoo.com.cn
Objective:
Our previous study shows that PURNE2 mRNA plays an important role in the differential diagnosis of leiomyosarcoma and gastrointestinal stromal tumor (GIST). Non-coding RNA PCA3 locates in the intron of PRUNE2 and may play a role in PRUNE2 expression. The aim of this study was to explore the expression of PCA3 mRNA and PRUNE2 in leiomyosarcoma and their correlation.
Methods:
The expression of PRUNE2 mRNA was analyzed by agilent gene expression microarray CHIP in 31 leiomyosarcomas and 37 GISTs, and the correlation of the PRUNE2 expression and prognosis of leiomyosarcoma was predicted. Real-Time PCR assay was used to detect the mRNA levels of PCA3 and PRUNE2 in 13 leiomyosarcomas and to investigate their correlation. Seven prostate cancer tissues were used as control of PCA3.
Results:
The level of PRUNE2 mRNA expression was significantly higher in the 31 leiomyosarcomas than that in the 37 GISTs, and the level of PRUNE2 mRNA expression was correlated with survival of the leiomyosarcoma patients. Compared with prostate cancer, the non-coding RNA PCA3 expression level was significantly lower in leiomyosarcoma, and it had no correlation with the prognosis of leiomyosarcoma. Most importantly, the PRUNE2 and PCA3 mRNA expressions were both upregulated in leiomyosarcoma and showed a significant positive correlation.
Conclusions:
Our findings demonstrate for the first time that PRUNE2 expression is correlated with the survival of leiomyosarcoma patients. Furthermore, non-coding RNA PCA3, which locates in the intron of PRUNE2, has a significant positive correlation with PRUNE2 and may play an important role in the pathogenesis of leiomyosarcoma.
Insights
PRUNE2 mRNA is upregulated in leiomyosarcoma and linked to patient survival. Non-coding RNA PCA3, found within PRUNE2, also increases in leiomyosarcoma and positively correlates with PRUNE2, suggesting a role in disease development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Context:
- Leiomyosarcoma and gastrointestinal stromal tumor (GIST) diagnosis can be challenging.
- Previous research indicated PRUNE2 mRNA's importance in differentiating these tumors.
- Non-coding RNA PCA3 is located within the PRUNE2 gene and may influence its expression.
Purpose:
- To investigate the expression levels of PCA3 mRNA and PRUNE2 in leiomyosarcoma.
- To determine the correlation between PCA3 and PRUNE2 expression in leiomyosarcoma.
- To explore the relationship between PRUNE2 expression and leiomyosarcoma patient prognosis.
Summary:
- PRUNE2 mRNA expression was significantly higher in leiomyosarcoma compared to GIST.
- PRUNE2 mRNA levels correlated with leiomyosarcoma patient survival.
- PCA3 mRNA expression was lower in leiomyosarcoma than in prostate cancer but showed a significant positive correlation with PRUNE2 in leiomyosarcoma.
Impact:
- This study establishes PRUNE2 expression as a prognostic marker for leiomyosarcoma.
- The findings highlight a significant positive correlation between PRUNE2 and PCA3 mRNA in leiomyosarcoma.
- PCA3 may play a crucial role in the pathogenesis of leiomyosarcoma, warranting further investigation.
