Related Experiment Video
Updated: May 18, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Preparation and characterization of neutral poly(ethylene glycol) methacrylate-based monolith for normal phase liquid
Yun Li1, Milton L Lee, Jing Jin
1Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, PR China.
Abstract:
A novel porous poly(ethylene glycol) methacrylate-based monolithic column for normal phase liquid chromatography was prepared by thermally initiated polymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EDMA) in the presence of selected porogens. The monolith was macroscopically homogeneous, had low flow resistance, and did not swell or shrink significantly in solvents of different polarities. Inverse size-exclusion data indicate that the monolith had a total porosity of 79.2%, including an external porosity of 69.3% and an internal porosity of 9.9%. Due to its mild polarity (hydrophilicity), the PEG-functionalized monolith could perform traditional normal phase chromatography using non-polar solvents The van Deemter plot demonstrated that the column efficiency of 33,600-34,320 theoretical plates/m could be achieved at a linear flow velocity of 0.9-1.5mm/s. The dual retention capability (both weak hydrophilic and hydrophobic interactions) investigated in this paper explains well why the PEG-functionalized monolith could operate in various chromatographic modes.
More Related Videos
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
High-Performance Liquid Chromatography: Elution Process
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...

