In-situ differential pulse anodic stripping voltammetry combined with hollow fiber-based liquid-three phase micro...

A new method has been proposed based on hollow fiber-based liquid three-phase micro extraction and in-situ differential pulse anodic stripping voltammetry (DPASV) for the micro extraction and quantification of mercury(II) ions. Different factors affecting the liquid-three phases micro extraction, including organic solvent, pH of the donor and acceptor phases, concentration of the complexing agent, extraction time, and stirring rate were investigated and the optimal extraction conditions were established. Three microelectrodes designed and constructed for this study were inserted into the two ends of a hollow fiber inside the acceptor solution, and then voltammetric analysis was performed in-situ during the extraction time. After 1600 s, final stable signal was used for the analytical applications. Under the optimized conditions, an enrichment factor of 277 was achieved and the relative standard deviation (R.S.D.) of the method was 6.2% (n=5). The calibration curve was obtained in the range of 0.2-30.0 nmol L(-1) Hg(II) with a reasonable linearity (R(2)>0.9880) and a limit of detection of 0.06 nmol L(-1). Finally, the applicability of the proposed method was evaluated by extraction and determination of mercury in real samples such as fish and rice.