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Developing improved immunoassays for paralytic shellfish toxins: the need for multiple, superior antibodies
Betsy Jean Yakes1, Samantha M Prezioso, Stacey L DeGrasse
1U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD 20740, USA. betsy.yakes@fda.hhs.gov
Talanta
|September 13, 2012
Summary
Developing a new assay using multiple antibodies can improve the detection of paralytic shellfish toxins (PSTs) in seafood. This approach enhances food safety and reduces economic losses in shellfish harvesting.
Area of Science:
- Food Science
- Analytical Chemistry
- Toxicology
Background:
- Paralytic shellfish toxins (PSTs) pose a significant risk to human health through seafood consumption.
- Accurate toxicity assessment is crucial for consumer protection and sustainable shellfish harvesting.
Purpose of the Study:
- To investigate the potential of combining multiple antibodies for a more accurate PST toxicity screening assay.
- To evaluate the reactivity profiles of three available antibodies using surface plasmon resonance (SPR).
Main Methods:
- Utilized surface plasmon resonance (SPR) biosensor assays to determine antibody reactivity profiles.
- Investigated the combined use of three antibodies with varying PST congener reactivities.
Main Results:
- Identified challenges in detecting R1-hydroxylated PSTs.
- Demonstrated that a multiplexed antibody approach may increase confidence in overall toxicity and toxin profiling.
Conclusions:
- Combining multiple antibodies in biosensor assays offers a promising strategy for improved PST detection.
- This multiplexed approach can be applied to lateral flow immuno-chromatographic platforms, potentially reducing reliance on animal bioassays and LC/MS.
