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Updated: May 18, 2026
11:53
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
Published on: July 12, 2017
The mammalian gene function resource: the International Knockout Mouse Consortium.
Allan Bradley1, Konstantinos Anastassiadis, Abdelkader Ayadi
1The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK. abradley@sanger.ac.uk
Summary
The International Knockout Mouse Consortium (IKMC) generated over 17,400 mutant mouse embryonic stem cell clones and 1,700 mutant strains. These resources significantly advance functional gene annotation and biomedical research.
Area of Science:
- Genomics
- Mammalian Genetics
- Molecular Biology
Background:
- The International Knockout Mouse Consortium (IKMC) was established in 2007 with the goal of creating mutations in nearly every protein-coding gene in the mouse genome.
- This initiative aimed to create a comprehensive resource for understanding gene function in mammals.
Purpose of the Study:
- To report on the progress and achievements of the IKMC 5 years after its inception.
- To highlight the development of high-throughput pipelines for generating mutant mouse models.
- To introduce the common IKMC web portal for accessing these valuable research materials.
Main Methods:
- Development of high-throughput gene trapping and gene-targeting pipelines.
- Generation of a large collection of mutant murine embryonic stem (ES) cell clones.
- Creation of numerous mutant mouse strains, with a focus on conditional mutations.
Main Results:
- Over 17,400 mutant murine ES cell clones have been generated.
- More than 1,700 mutant mouse strains have been created, predominantly conditional.
- A centralized IKMC web portal (www.knockoutmouse.org) was established for data accessibility.
Conclusions:
- The IKMC has successfully created an unparalleled resource of mutant mouse models.
- These materials significantly enhance the functional annotation of the mammalian genome.
- The IKMC resources are poised to have a major impact on future biomedical research and discovery.
Related Concept Videos
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...